DOT-ELISA and parasitological examination for diagnosis of Schistosoma mansoni infection in Nigeria
نویسندگان
چکیده
منابع مشابه
Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this...
متن کاملParasitological characteristics of Schistosoma mansoni infection in swiss mice with underlying malnutrition.
The effects of a protein-restricted diet (8% protein, 81% carbohydrate and 11% lipids) on Schistosoma mansoni infectivity, fecal egg excretion and intestinal egg distribution in Swiss (SW) mice were studied. Pregnant mice received a deficient diet from the middle of gestation until delivery. Seven-days-old mice were exposed to 50 cercariae (BH strain, Brazil). Offspring mice had a free access t...
متن کاملSimplification and standardization of dot-ELISA for human schistosomiasis mansoni.
Dot-ELISA, a technique that shares the same principles as the enzyme immunoassay, is useful for detection of anti-Schistosoma mansoni antibodies in the sera of patients with Schistosoma mansoni infections. The antigens were fixed to the nitrocellulose strips, blocked with 1% bovine serum albumin in 0.05% Tween 20. Patient sera (40) and normal laboratory personnel sera (9) were applied to the sh...
متن کاملdesigning and evaluation of dot-elisa for diagnosis of fasciola infection in cattle
fasciolosis is a disease caused by liver fluck of the genus of fasciola. diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. in this study, an in-house dot-elisa method; using excretion–secretory (es ag) and crude (cr ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. for this purpose, the sera...
متن کاملImmunopathology of Schistosoma mansoni infection.
Schistosomiasis mansoni is a chronic helminthic disease that affects about 100 million people in the tropics. The worms have a life span of 5 to 10 years, and they live in the mesenteric veins of the host. Lightly infected individuals are asymptomatic or manifest mild intestinal symptoms. Heavily infected individuals often develop severe morbidity with hepatosplenomegaly, sometimes with a fatal...
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ژورنال
عنوان ژورنال: Helminthologia
سال: 2006
ISSN: 1336-9083,0440-6605
DOI: 10.2478/s11687-006-0003-3